Cytogenetics witnessed, in recent years, a series of technological innovation that forced the field to completely and repeatedly renew its approach and vision of chromosomal imbalances and copy number variation in the population, in cancer and in evolution. Molecular cytogenetics, microarray-CGH, and massive sequencing technologies are the most relevant techniques that trigged these changes.

The Course is designed to give a broad overview of these technologies and to discuss with attendants the way they integrate each other in a modern genetic/cytogenetic laboratory.

Antonio Baldini (Naples, Italy)

Mariano Rocchi (Bari, Italy)

Felix Mitelman (Lund, Sweden)

Brian Young (London, UK)

Giuseppe Calabrese (Chieti, Italy)

Wei Chen (Berlin, Germany)

John Crolla (Salisbury, UK)

Gianluca De Bellis (Segrate, Italy)

Peter Robinson (Berlin, Germany)

Frank Speleman (Ghent, Belgium)

Joris Vermeesch (Leuven, Belgium)

Bauke Ylstra (Amsterdam, The Netherlands)

Orsetta Zuffardi (Pavia, Italy)

Saturday, October 16th

Morning session: Introduction to classic cytogenetics

08:30: Registration and Overview of the course

09:00: O. Zuffardi, Cytogenetics

09:45: F. Mitelman, Cancer cytogenetics

10:30: Coffee break

11:00: M. Rocchi, Human genome plasticity

11:45: J. Crolla, t.b.d.

12:30: Questions from students on morning lectures

13:00: Lunch break

Afternoon session: 14:30 - 18:00

14:30-15:00: Nugen Technologies: Linear amplification of  RNA and DNA for microarray and NGS analysis
Concurrent Workshops

Sunday, October 17th

Morning session: Molecular Cytogenetics

09:00: J. Crolla, t.b.d.

09:45: O. Zuffardi, CNV in clinics

10:30: Coffee break

11:00: G. Calabrese, FiberFISH

11:45: B. Young, The application of SNP arrays to the cancer genome

12:30: Questions from students on morning lectures

13:00: Lunch break

Afternoon session: 14:30 - 18:00

Concurrent Workshops

Monday,  October 18th: Biomedical and Clinical Research

Morning session:

09:00: J. Vermeesch, Genome wide CNV and SNP detection in single cells

09:45: F. Speleman, QPCR platform for miRNA

10:30: Coffee break

11:00: B. Young, Expression profiling by microarray

11:45: A. Baldini, t.b.d.

12:30: Questions from students on morning lectures

13:00: Lunch break

Afternoon session: 14:30 - 18:00

Concurrent Workshops

Tuesday, October 19th: Massive Sequencing

Morning session:

09:00: B. Ylstra, Technology advancement: from microarrays to deep sequencing

09:45: W. Chen, Solexa sequencing technology

10:30: Coffee break

11:00: G. De Bellis, 454 sequencing technology

11:45: J. Vermeesch, The impact of massive sequencing on genetic research

12:30: Questions from students on morning lectures

13:00: Lunch break


Wednesday, October 20t:

Morning session: Ontology approach to phenotype classification

09:00: W. Chen, Application of Illumina/Solexa sequencing technology in genetic research

09:45: G. De Bellis, Application of 454 sequencing technology in genetic research

10:30: Coffee break

11:00: P. Robinson, Identity-by-Descent Filtering of Exome Sequence Data

12:30: Questions from students on morning lectures

13:00: Lunch break

Departure after lunch