The `Practical Workshop on Thalassemia` is dedicated to MEDA country participants only! The participation is limited to 15 attendees and the fee will be covered by the Eumedis program fellowships, granted by the European Commission.
Laboratory practical workshop:
Practical Workshop on Thalassemia
March 18-20, 2004
Thursday, March 18th
9.00-9.30 Opening introduction to the workshop
9.30-13.00 Practical work: PCR reactions
10.30-11.30 Lecture "Introduction to DNA array technology"
14.15-15.00 Practical work: Control of PCR reactions by agarose gel electrophoresis and pooling the PCR reaction products
15.00-16.00 Practical work: Purification and concentration of pooled PCR products
16.00-17.00 Practical work: Control of purified and concentrated PCR products by agarose gel electrophoresis
17.15-18.00 Discussion of the day
Friday, March 19th
9.00-11.00 Practical work: Fragmentation of PCR products by sAP and UNG treatment.
9.30-10.30 Lecture "Arrayed Primer Extension (APEX) technology as a tool for mutation detection, genotyping and DNA resequencing
11.00-11.30 Practical work: Control of DNA fragmentation by electrophoresis
11.45-13.00 Practical work: APEX reactions
14.15-16.00 Practical work: Imaging of APEX slides.
15.00-15.45 Lecture "Imaging of APEX slides by Genorama Quattroimager"
16.00-18.00 Practical work: Analysis of APEX reactions by Genorama software
18.00-19.00 Discussion of the day - Conclusions
Saturday, August 28th
9.00-12.00 Discussions and conclusions
APEX on DNA microarrays: applications in SNP analysis, mutation detection and DNA resequencingEuropean Genetics Foundation/Asper Biotech Ltd.August 26 - September 1, 2002, Tartu EstoniaMarch18-20, 2004, Bologna, Italy