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From2004-03-18
To2004-03-20
Course

The `Practical Workshop on Thalassemia` is dedicated to MEDA country participants only! The participation is limited to 15 attendees and the fee will be covered by the Eumedis program fellowships, granted by the European Commission.

 Laboratory practical workshop:

Practical Workshop on Thalassemia
March 18-20, 2004
Bologna, Italy


PROGRAMME:


Thursday, March 18th



9.00-9.30 Opening introduction to the workshop

9.30-13.00 Practical work: PCR reactions

10.30-11.30 Lecture "Introduction to DNA array technology"

13.00-14.00 Lunch

14.15-15.00 Practical work: Control of PCR reactions by agarose gel electrophoresis and pooling the PCR reaction products

15.00-16.00 Practical work: Purification and concentration of pooled PCR products

16.00-17.00 Practical work: Control of purified and concentrated PCR products by agarose gel electrophoresis

17.15-18.00 Discussion of the day



Friday, March 19th



9.00-11.00 Practical work: Fragmentation of PCR products by sAP and UNG treatment.

9.30-10.30 Lecture "Arrayed Primer Extension (APEX) technology as a tool for mutation detection, genotyping and DNA resequencing

11.00-11.30 Practical work: Control of DNA fragmentation by electrophoresis

11.45-13.00 Practical work: APEX reactions

13.00-14.00 Lunch

14.15-16.00 Practical work: Imaging of APEX slides.

15.00-15.45 Lecture "Imaging of APEX slides by Genorama Quattroimager"

16.00-18.00 Practical work: Analysis of APEX reactions by Genorama software

18.00-19.00 Discussion of the day - Conclusions



Saturday, August 28th



9.00-12.00 Discussions and conclusions
APEX on DNA microarrays: applications in SNP analysis, mutation detection and DNA resequencingEuropean Genetics Foundation/Asper Biotech Ltd.August 26 - September 1, 2002, Tartu EstoniaMarch18-20, 2004, Bologna, Italy

VenueDept. of Medical Genetics, University of Bologna
Directors

Ants Kurg (Estonia)

Speakers

Hanno Roomere (Estonia), Chiara Perra (Cagliari, Italia)

Programme
European School of Genetic Medicine